MyD88依赖途径在膝骨关节炎进程中的基因表达相关性研究 |
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投稿时间:2017-03-11 修订日期:2018-04-07
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期刊信息:《中国骨伤》年,第卷,第期,第-页 |
DOI: |
基金项目:国家自然科学基金项目(面上项目,重点项目,重大项目) |
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中文摘要:目的:探讨MyD88依赖途径的相关基因TLR4、NF-κB与MyD88在膝骨关节炎(Osteoarthritis,OA)不同病程下大鼠滑膜组织的表达特点及相关性。方法:将60只Wistar大鼠随机分为6组:空白组(N)、假手术组(F)和模型组(2周组(2W)、4周组(4W)、8周组(8W)、12周组(12W)),各10只。各模型组以Hulth法建立大鼠膝OA动物模型,空白组不予手术,假手术组仅打开关节腔。按上述设计时间收集样本,经总RNA的提取及反转录后,用Real-time PCR法对各组滑膜组织中的TLR4、NF-κB及MyD88的相对表达量进行检测,并用统计软件计算其相关性。结果:与空白组相比,假手术组TLR4、MyD88、NF-κB的mRNA相对表达量均无显著性差异(P>0.05),说明手术造模对结果无明显影响;而各模型组相较于空白组,各基因表达增强,有统计学意义(P<0.05)。相关性分析显示:MyD88与TLR4及NF-κB的mRNA相关性系数分别为0.91和0.86,差异有统计学意义(P<0.05)。结论:MyD88与TLR4、NF-κB的表达均呈显著的正相关性,在通路上起重要的枢纽作用,可通过其表达预测通路上其他基因的表达情况,进一步明确膝OA病理机制。 |
【关键词】膝骨关节炎 MyD88依赖途径 固有免疫 滑膜炎症 |
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Research on the Correlation of the Genes Expression Related to MyD88-dependent Pathway in Progression of KneeSOsteoarhritis |
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ABSTRACT Objective: To investigate the expression features and the correlation of the genes related to MyD88-dependent pathway in synovial membrane (SM) of progression of knee osteoarthritis (OA). Methods: 60 Wistar rats were divided into 6 groups randomly: the blank group (N), the false surgical group (F), The model groups (2 weeks (2W), 4 weeks (4W), 8 weeks (8W) and 12 weeks (12W)), and each group had 10 rats. The models were intervened by using Hulth method. N group experienced no surgery, while F group was only opened the joint cavity and sutured. The SM samples were collected according to the time designed above. The relative expression quantity of MyD88, TLR4 and NF-κB was detected by Real-time PCR after extracted the total RNA and reverse transcription. The correlation analysis was obtained by SPSS. Results: There was no significant difference in the each gene mRNA expression in F group, compared with N group(P>0.05), while enhanced expression was found in the model groups(P<0.05). The correlation analysis showed that r=0.91 between MyD88 and TLR4(P<0.05), and r=0.86 between MyD88 and NF-κB(P<0.05). Conclusion: Positively relative between MyD88/TLR4 and MyD88/NF-κB. MyD88 played a central hub role in TLR4/NF-κB signal passway, by which we could predicte the expression of other genes in the passway. It also could provide the basis for further clarify the pathologic mechanism of knee OA. |
KEY WORDS Knee osteoarthritis, MyD88-dependent pathway, InnateSimmunity, Synovitis |
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