风寒湿刺激对家兔颈椎间盘MMP-1、MMP-3活性的影响
摘要点击次数: 1561   全文下载次数: 828   投稿时间:2001-09-25    
作者Author单位AddressE-Mail
施杞 SHI Qi 上海中医药大学附属龙华医院,上海200032 Longhua Hospital,Shanghai University of TCM Shanghai,200032  
王拥军 WANG Yong jun 上海中医药大学附属龙华医院,上海200032 Longhua Hospital,Shanghai University of TCM Shanghai,200032  
吴士良 WU Shi liang 苏州大学医学院,江苏苏州  
葛京化 上海中医药大学附属龙华医院,上海200032 Longhua Hospital,Shanghai University of TCM Shanghai,200032  
杨阳 上海中医药大学附属龙华医院,上海200032 Longhua Hospital,Shanghai University of TCM Shanghai,200032  
刘梅 上海中医药大学附属龙华医院,上海200032 Longhua Hospital,Shanghai University of TCM Shanghai,200032  
周重建 上海中医药大学附属龙华医院,上海200032 Longhua Hospital,Shanghai University of TCM Shanghai,200032  
期刊信息:《中国骨伤》2002年,第15卷,第6期,第340-342页
DOI:doi:10.3969/j.issn.1003-0034.yyyy.nn.zzz
基金项目:国家自然科学基金项目(No.39970917)
中文摘要:

目的:在建立不同强度风寒湿痹证型颈椎病动物模型的基础上,检测实验家兔颈椎间盘组织中胶原酶(MMP1)、中性蛋白多糖酶(MMP3)活性变化。

方法:8月龄雄性新西兰白兔24只,随机分为正常对照组和轻度、中度、重度刺激组。采用刺激条件是温度5℃±0.5℃,湿度100%,风力6级,按轻、中、重度刺激组的不同要求,分别给予32、64和128小时的间断重复刺激,每日刺激4小时。分别采用3MH-Ⅰ型胶原蛋白法和免疫一步夹心法检测MMP-1、MMP-3活性。

结果:各个模型组退变指数都有不同程度上升,重度刺激组较正常对照组有统计学差异(P<0.05)。模型组与对照组比较,中、重度刺激组MMP-1和MMP-3活性升高明显(P<0.01),轻度刺激组变化不明显;模型组之间比较,中、重度刺激组MMP-1、MMP-3活性明显高于轻度刺激组(P<0.01)。

结论:中、重度风寒湿刺激家兔颈椎间盘退变后使MMP1和MMP3活性升高,加速椎间盘细胞外基质降解,导致颈椎间盘退变。
【关键词】颈椎病  痹证  胶原酶  中性蛋白多糖酶
 
Effect on activity change of MMP-1,MMP-3 of cervical intervertebral discs of rabbits by irritancy of Wind-Cold-Dampness factors
ABSTRACT  

Objective:To establish cervical spondylosis animal models of Wind Cold Dampness arthralgia syndrome type.The morphologic state of the discs of the rabbits was observed with light microscope,and the activity of metalloproteinase 1,metalloproteinase 3 in the discs was assayed with biochemical.

Methods:Twenty four 8 months old rabbits were randomly allocated into four groups of the control,light,medium and heavy irritancy groups with 6 animals in each.The basic condition of Wind Cold Dampness is 6 class force of wind, 5℃±0.5℃ and 100% humidity.32,64,128 hours of continuous irritancy were applied on light,medium and heavy irritancy group respectively.The daily period of irritancy was 4 hour.

Results:The degree index number of intervertebral discs degeneration was elevated in every irritancy group,and in the heavy irritancy group the increase was significantly higher(P<0.05).MMP 1 and MMP 3 activities of cervical intervertebral discs in middle and heavy irritancy group,MMP 1 and MMP 3 activity in medium and heavy irritancy group were significantly higher than that in the control group respectively(P<0.01).

Conclusion:MMP 1 and MMP 3 activity were increased by medium and heavy Wind Cold Dampness irritancy.Acceleration of degradation of extracelluar matrix of cervical intervertebral discs resulted in degeneration of cervical intervertebral discs.
KEY WORDS  Cervical syndrome  Arthralgia syndrome  Metalloproteinases Ⅰ  Metalloproteinases Ⅲ
 
引用本文,请按以下格式著录参考文献:
中文格式:施杞,王拥军,吴士良,葛京化,杨阳,刘梅,周重建.风寒湿刺激对家兔颈椎间盘MMP-1、MMP-3活性的影响[J].中国骨伤,2002,15(6):340~342
英文格式:SHI Qi,WANG Yong jun,WU Shi liang.Effect on activity change of MMP-1,MMP-3 of cervical intervertebral discs of rabbits by irritancy of Wind-Cold-Dampness factors[J].zhongguo gu shang / China J Orthop Trauma ,2002,15(6):340~342
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