| 基于单细胞测序技术探讨脊髓损伤后星形胶质细胞异质性的研究进展 |
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Received:September 18, 2024
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| 作者 | Author | 单位 | Unit | E-Mail |
| 杜磊 |
DU Lei |
甘肃中医药大学, 甘肃 兰州 730000 |
Gansu University of Traditional Chinese Medicine, Lanzhou 730000, Gansu, China |
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| 张彦军 |
ZHANG Yan-jun |
甘肃省中医院, 甘肃 兰州 730050 |
Gansu Provincial Hospital of Traditional Chinese Medicine, Lanzhou 730050, Gansu, China |
1486815305@qq.com |
| 郭铁峰 |
GUO Tie-feng |
甘肃省中医院, 甘肃 兰州 730050 |
Gansu Provincial Hospital of Traditional Chinese Medicine, Lanzhou 730050, Gansu, China |
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| 罗林钊 |
LUO Lin-zhao |
甘肃省中医院, 甘肃 兰州 730050 |
Gansu Provincial Hospital of Traditional Chinese Medicine, Lanzhou 730050, Gansu, China |
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| 马平怡 |
MA Ping-yi |
甘肃省中医院, 甘肃 兰州 730050 |
Gansu Provincial Hospital of Traditional Chinese Medicine, Lanzhou 730050, Gansu, China |
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| 李家明 |
LI Jia-ming |
甘肃省中医院, 甘肃 兰州 730050 |
Gansu Provincial Hospital of Traditional Chinese Medicine, Lanzhou 730050, Gansu, China |
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| 檀盛 |
TAN Sheng |
甘肃中医药大学, 甘肃 兰州 730000 |
Gansu University of Traditional Chinese Medicine, Lanzhou 730000, Gansu, China |
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| 期刊信息：《中国骨伤》2025年38卷，第5期，第544-548页 |
| DOI：10.12200/j.issn.1003-0034.20240352 |
| 基金项目:国家自然科学基金地区项目(编号:82360947) |
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| 单细胞转录组测序技术在脊髓损伤(spinal cord injury,SCI)后星形胶质细胞(astrocytes)异质性的研究为创伤后神经再生和修复提供了新的视角。就单细胞测序技术在脊髓损伤后星形胶质细胞的研究进展进行综述,更加全面、深入地阐述单细胞测序技术在脊髓损伤后星形胶质细胞领域的应用。单细胞测序技术可以高通量地分析单个细胞的转录组,从而揭示细胞类型和状态的精细差异。通过使用单细胞测序技术,揭示SCI后星形胶质细胞的异质性及其与神经再生和修复的关联。总之,单细胞测序技术的应用为揭示SCI后星形胶质细胞异质性的研究提供了重要工具,进一步探索星形胶质细胞在SCI中的作用机制,并开发针对其调控机制的干预策略,以提高SCI的治疗效果。发现星形胶质细胞转录组动态变化提高了研究者们对脊髓损伤病变进程的理解,并为脊髓损伤在不同时间点的治疗提供了新见解。截至目前,这些研究结果均还需要更多的基础研究和足够的临床试验来验证。在未来,单细胞测序技术通过与生物信息学、计算机科学、组织工程学和临床医学等跨学科合作,有望为脊髓损伤的诊疗打开新的一扇窗户。 |
| [关键词]:单细胞测序技术 星形胶质细胞 脊髓损伤 神经恢复 病理机制 |
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| Progress in investigating astrocyte heterogeneity after spinal cord injury based on single-cell sequencing technology |
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| Abstract:In recent years,the study of single-cell transcriptome sequencing technology in the heterogeneity of astrocytes (astrocytes) after spinal cord injury (SCI) has provided new perspectives on post-traumatic nerve regeneration and repair. To provide a review on the research progress of single-cell sequencing technology in astrocytes after spinal cord injury (SCI),and to more comprehensively and deeply elaborate the application of single-cell sequencing technology in the field of astrocytes after SCI. Single-cell sequencing technology can analyse the transcriptomes of individual cells in a high-throughput manner,thus revealing fine differences in cell types and states. By using single-cell sequencing technology,the heterogeneity of astrocytes after SCI and their association with nerve regeneration and repair were revealed. In conclusion,the application of single-cell sequencing technology provides an important tool to reveal the heterogeneity of astrocytes after SCI,to further explore the mechanisms of astrocytes in SCI,and to develop intervention strategies targeting their regulatory mechanisms in order to improve the therapeutic efficacy of SCI. The discovery of changes in astrocyte transcriptome dynamics has improved researchers' understanding of spinal cord injury lesion progression and provided new insights into the treatment of spinal cord injury at different time points. To date,all of these findings need to be validated by more basic research and sufficient clinical trials. In the future,single-cell sequencing technology,through interdisciplinary collaboration with bioinformatics,computer science,tissue engineering,and clinical medicine,is expected to open a new window for the treatment of spinal cord injury. |
| KEYWORDS:Single cell sequencing technology Astrocytes Spinal cord injury Neurological recovery Pathological mechanism |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 杜磊,张彦军,郭铁峰,罗林钊,马平怡,李家明,檀盛.基于单细胞测序技术探讨脊髓损伤后星形胶质细胞异质性的研究进展[J].中国骨伤,2025,38(5):544~548 |
| 英文格式: | DU Lei,ZHANG Yan-jun,GUO Tie-feng,LUO Lin-zhao,MA Ping-yi,LI Jia-ming,TAN Sheng.Progress in investigating astrocyte heterogeneity after spinal cord injury based on single-cell sequencing technology[J].zhongguo gu shang / China J Orthop Trauma ,2025,38(5):544~548 |
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