银杏叶提取液对缺血再灌注骨骼肌线粒体的保护作用
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作者Author单位AddressE-Mail
蓝旭 LAN Xu 兰州军区总医院骨科研究所,甘肃兰州730050 Institute of Orthopedics,The General Hospital of Lanzhou Military Command Gansu Lanzhou,730050  
刘雪梅 LIU Xue mei 兰州军区总医院骨科研究所,甘肃兰州730050 Institute of Orthopedics,The General Hospital of Lanzhou Military Command Gansu Lanzhou,730050  
葛宝丰 GE Bao feng 兰州军区总医院骨科研究所,甘肃兰州730050 Institute of Orthopedics,The General Hospital of Lanzhou Military Command Gansu Lanzhou,730050  
许建中 第三军医大学西南医院,重庆  
期刊信息:《中国骨伤》2002年,第15卷,第6期,第347-348页
DOI:doi:10.3969/j.issn.1003-0034.yyyy.nn.zzz
基金项目:
中文摘要:

目的:观察银杏叶提取液对肢体缺血再灌注损伤的影响。

方法:制作兔肢体缺血再灌注损伤动物模型,实验分对照组、再灌注组和治疗组。检测骨骼肌三磷酸腺苷(ATP)水平并观察线粒体超微结构的变化,测定线粒体丙二醛(MDA)、还原型谷胱甘肽(GSH)和线粒体Ca含量。

结果:再灌注组和对照组比较,以上各项指标差异显著(P<0.01,P<0.05)。银杏叶提取液促进骨骼肌能量代谢、维持线粒体结构完整性、降低线粒体MDA含量、提高线粒体GSH水平并抑制线粒体Ca超载。治疗组各项测定指标较再灌注组比较明显改善(P<0.01,P<0.05)。

结论:银杏叶提取液对缺血再灌注骨骼肌线粒体有保护作用。
【关键词】肢体  再灌注损伤  线粒体  骨胳肌  中医现代化
 
Protective effect of Ginkgo Biloba succi on mitochondria of skeletal muscle during ischemia and reperfusion injury
ABSTRACT  

Objective:To observe the effect of Ginkgo Biloba succi on ischemia and reperfusion injury of Limb

Methods:The experimental model of ischemia and reperfusion injury of limb was produced in rabbits which were divided into control group,reperfusion group and treatment group.The changes of ATP synthesis and mitochondrial ultrastructure were observed,while the contents of MDA,GSH and Ca in mitochondria were measured respectively.

Results:The indexes of reperfusion group changed significantly as compared with those of control group(P<0.01,P<0.05).Ginkgo Biloba succi promoted the recovery of energy metabolism,maintained the integrality in mitochondrial structure,reduced the mintochondrial MDA content,increased the level of GSH,inhibited the calcium overload.The indexes of treatment group improved significantly as compared with those of reperfusion group(P<0.01,P<0.05).

Conclusion:Ginkgo Biloba succi might have the protective effect on muscle cells during ischemia and reperfusion injury of limb.
KEY WORDS  Limb  Reperfusion injury  Mitochondria  Skeletal muscle  Modernization of TCM
 
引用本文,请按以下格式著录参考文献:
中文格式:蓝旭,刘雪梅,葛宝丰,许建中.银杏叶提取液对缺血再灌注骨骼肌线粒体的保护作用[J].中国骨伤,2002,15(6):347~348
英文格式:LAN Xu,LIU Xue mei,GE Bao feng.Protective effect of Ginkgo Biloba succi on mitochondria of skeletal muscle during ischemia and reperfusion injury[J].zhongguo gu shang / China J Orthop Trauma ,2002,15(6):347~348
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