| 补肾活血方对离体胎鼠颅骨成骨细胞增殖的影响 |
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投稿时间：2002-08-26 修订日期：2003-01-02
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| 期刊信息：《中国骨伤》2003年，第16卷，第4期，第211-212页 |
| DOI：doi:10.3969/j.issn.1003-0034.yyyy.nn.zzz |
| 基金项目:湖北省卫生科研基金(W9721);湖北省自然科学基金(99J136) |
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中文摘要:
目的:探讨补肾活血法对成骨细胞代谢的影响。
方法:胎鼠颅骨成骨细胞培养于含有0,10,100,1000,5 000μg.ml-15种浓度的补肾活血方药液培养基中72h后,分别采用噻唑篮比色法和胸腺嘧啶核苷渗入法检测成骨细胞增殖、分化情况,并与具有促细胞增殖作用的成纤维细胞生长因子作对照。
结果 :补肾活血方药液以剂量依赖方式促进成骨细胞的增殖,与对照组相比,100 g.d-‘药液即可促进成骨细胞增殖,并在1000μg.ml-1浓度时达到最大效应（P<0.05,P<0.01）。
结论:补肾活血方具有促进骨形成作用。 |
| 【关键词】成骨细胞 动物,实验 中草药 |
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| Effects of Chinese medicine Bushenhuoxue on the proliferation of fetal rat calvarial osteoblast in vitro |
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ABSTRACT
Objective:To investigate the effects of Bushenghuoxue on osteoblastic cell metabolism
Methods:Fetal rat calvarial osteoblastic cell were cultured in the medium containing Bushenghuoxue with five different concentrations,0,10,100,1 000,and 5 000μg·ml-1 ,and incubated for 72 hours.Calorimetric MMT,3H-thymidine incorporation were used to determine the condition of cell proliferation and differentiation in vitro.
Results:Bushenghuoxue with 100 μg·ml-1 might promoted the proliferation of osteoblastic cells. Its effects was concentration-dependent. 1000 μg·ml-1 may reach the highest effect.
Conclusion:Bushenghuoxue can promote the bone formation. |
| KEY WORDS Osteoblasts Animals, laboratory Drugs, Chinese herbal |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 汤耿民,沈霖,肖明英.补肾活血方对离体胎鼠颅骨成骨细胞增殖的影响[J].中国骨伤,2003,16(4):211~212 |
| 英文格式: | TANG Gengmin,SHEN Lin,XIAO Mingying.Effects of Chinese medicine Bushenhuoxue on the proliferation of fetal rat calvarial osteoblast in vitro[J].zhongguo gu shang / China J Orthop Trauma ,2003,16(4):211~212 |
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