| 二磷酸盐对关节磨屑刺激单核细胞分泌IL-1β的抑制作用 |
|
摘要点击次数: 1718
全文下载次数: 990
投稿时间：2003-12-30
|
|
|
| 期刊信息：《中国骨伤》2004年，第17卷，第10期，第596-598页 |
| DOI：doi:10.3969/j.issn.1003-0034.yyyy.nn.zzz |
| 基金项目: |
|
中文摘要:
目的:研究二磷酸盐对关节磨屑刺激单核细胞分泌IL-β的影响。
方法:分离培养人外周血单核细胞,加入关节磨屑及不同浓度的阿伦膦酸钠,ELISA检测细胞上清中IL-1β的含量,原位杂交检测IL-1βmRNA的表达。
结果:关节磨屑组IL-1β及基因的表达显著高于对照组(P<0.01),不同浓度的阿伦膦酸钠组IL-1β及基因的表达明显低于关节磨屑组(P<0.01)。
结论:阿伦膦酸钠能通过下调人外周血单核细胞IL-1β3及mRNA的表达,对人工关节松动可能起防治作用。 |
| 【关键词】二磷酸盐 关节磨屑 白细胞介素-1 |
| |
| Inhibitory effect of bisphosphonates on IL-1β expression of human monocytes induced by wear debris |
|
ABSTRACT
Objective:To study the effect of bisphosphonates on IL-1β expression of human monocytes with wear particles stimulation.
Methods: In vitro human peripheral blood mononuclear cell (PBMC) were cultured with wear particles and bisphosphonates of different concentration, then productions of IL-1β and IL-1β mRNA were determined by ELISA and in situ hybridization techniques.
Results:The production of IL-1β were increased significantly when wear particles was added,compared with control group (P<0.01). And bisphosphonates reduced the level of IL-1β. Hybridization demonstrated bisphosphonates might down regulate IL-1β mRNA expression.
Conclusion:Bisphosphonates can inhibit IL-1β expression induced by wear debris and may be used in the prevention and treatment of aseptic loosening of prosthesis. |
| KEY WORDS Bisphasphonates Wear debris Interleukin-1 |
| |
| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 陈明,夏仁云.二磷酸盐对关节磨屑刺激单核细胞分泌IL-1β的抑制作用[J].中国骨伤,2004,17(10):596~598 |
| 英文格式: | CHEN Ming,XIA Ren-yun.Inhibitory effect of bisphosphonates on IL-1β expression of human monocytes induced by wear debris[J].zhongguo gu shang / China J Orthop Trauma ,2004,17(10):596~598 |
|
| 阅读全文 下载 查看/发表评论 下载PDF阅读器 |
| 关闭 |
