| 中药黄芪对脊髓星形胶质细胞单核细胞趋化蛋白-1分泌的影响 |
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投稿时间：2004-01-13
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| 期刊信息：《中国骨伤》2004年，第17卷，第11期，第653-655页 |
| DOI：doi:10.3969/j.issn.1003-0034.yyyy.nn.zzz |
| 基金项目: |
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中文摘要:
目的:研究黄芪对体外培养的脊髓星形胶质细胞趋化蛋白1(MCP-1)分泌的影响。
方法:自Wistar大鼠脊髓组织分离、纯化星形胶质细胞,体外培养,分别予以TNF-α及TNFα+黄芪处理,ELISA方法检测培养上清液中MCP-1的表达。
结果:TNF-α可以显著刺激星形胶质细胞合成、释放MCP-1,而黄芪可下调其刺激作用。
结论:受炎症因子刺激活化的星形胶质细胞可能是损伤脊髓局部MCP-1的来源之一,黄芪可以减少脊髓损伤后内源性MCP-1的产生,从而缓解继发性脊髓损伤,发挥脊髓保护作用。 |
| 【关键词】黄芪 单核细胞趋化蛋白-1 脊髓损伤 星形胶质细胞 |
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| Effects of astragalus root on the secretion of MCP-1 by astrocytes in vitro from spinal cord of adult rats |
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ABSTRACT
Objective:To study the secretion of MCP-1 by astrocytes culturted in vitro and the possible influence of astragalus root(AR).
Methods:Astrocytes were separated and purified from spinal cord of adult Wistar rats.The expression of MCP-1 was detected by ELISA when the astrocyte were culturted in vitro with TNF-α or combined with AR.
Results:TNF-α markedly increase the secretion of astrocyte-derived MCP-1 which could be down-regulated by AR to some extent.
Conclusion:Astrocytes activated by some inflammatory factors may be one of the sources of MCP-1 secreted in the local injuried spinal cord.AR probably can decrease the engenous expression of MCP-1 and thus show a neuroprotective effects. |
| KEY WORDS Astragalus root Monocyte chemoattractant protein-1(MCP-1) Spinal injuries Astrocyte |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 马永刚,刘世清,刘敏,彭昊.中药黄芪对脊髓星形胶质细胞单核细胞趋化蛋白-1分泌的影响[J].中国骨伤,2004,17(11):653~655 |
| 英文格式: | MA Yong-gang,LIU Shi-qing,LIU Min,PENG Hao.Effects of astragalus root on the secretion of MCP-1 by astrocytes in vitro from spinal cord of adult rats[J].zhongguo gu shang / China J Orthop Trauma ,2004,17(11):653~655 |
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