| 补肾壮筋汤对兔早期实验性膝骨关节炎软骨细胞凋亡及增殖细胞核抗原表达的影响 |
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投稿时间：2004-05-10
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| 期刊信息：《中国骨伤》2005年，第18卷，第5期，第278-281页 |
| DOI：doi:10.3969/j.issn.1003-0034.yyyy.nn.zzz |
| 基金项目: |
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中文摘要:
目的:探讨以补肝益肾为治疗手段的补肾壮筋汤对兔早期膝关节实验性OA的软骨细胞凋亡和增殖细胞核抗原(PCNA)表达的影响。
方法:40只青紫蓝兔随机分为4组,每组10只,分别为中药组、假模组、模型组、正常组,参照Hulth法造成早期骨关节炎模型。术后第4周开始,中药组、假模组口服补肾壮筋汤,模型组、正常组口服生理盐水。7周末取兔膝关节软骨,肉眼、光镜、透射电镜观察形态学变化,用原位末端标记法(TUNEL法),免疫组化法分别观察软骨细胞凋亡和PCNA的表达。
结果:中药组的软骨细胞凋亡指数小于模型组(P<0.05);而中药组的PCNA表达要高于其他各组(P<0.05)。
结论：补肾壮筋汤减少兔早期膝关节实验性OA软骨细胞的凋亡,促进软骨细胞的增殖,对早期OA有治疗作用。 |
| 【关键词】补肾壮筋汤 骨性关节炎,膝细胞凋亡 细胞核增殖抗原 |
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| Effects of Bushen Zhuangjin decoction(补肾壮筋汤) on apoptosis of chondrocyte and expression of PCNA in earlier period experimental osteoarthritis of rabbit |
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ABSTRACT
Objective:To observe the effects of Bushen Zhuangjin decoction(补肾壮筋汤) on apoptosis of chondrocyte,expression of proliferation cell nuclear antigen(PCNA)in earlier period of experimental osteoarthritis of rabbit,and analyze the relation between of them.
Methods:Forty Livor rabbits were divided into four groups randomly:Normal group,model contrast group,Chinese herb group,sham operation group.There were ten rabbits in every group.OA model was induced in rabbits by Hulth's method.After three weeks Chinese herb group and sham operation group(which only cut out the skin)fed with Bushen Zhuangjin decoction,normol group and model contrast group fed with 0.9%NS at the same time.Specimens were harvested at the 8th week.Cartilage sections were analyzed by immunohistochemistry for the PCNA,TUNEL for apoptosis.Observe the change of morphology of chondrocyte by light microscopic and transmission electron microscopic.
Results:Apoptosis cell index and cell proliferation index have significant difference between Chinese herb group and model contrast group(P<0.05).
Conclusion:Bushen Zhuangjin decoction can decrease the apoptosis of chondrocyte,increase of proliferation chondrocyte.There was therapeutical effect in the earlier period of experimental osteoathritis. |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 潘浩,胡庆丰,李雄峰,边红光,章煜铭.补肾壮筋汤对兔早期实验性膝骨关节炎软骨细胞凋亡及增殖细胞核抗原表达的影响[J].中国骨伤,2005,18(5):278~281 |
| 英文格式: | PAN Hao,HU Qing-feng,LI Xiong-feng,BIAN Hong-guang,ZHANG Yu-ming.Effects of Bushen Zhuangjin decoction(补肾壮筋汤) on apoptosis of chondrocyte and expression of PCNA in earlier period experimental osteoarthritis of rabbit[J].zhongguo gu shang / China J Orthop Trauma ,2005,18(5):278~281 |
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