| 活血止痛汤对硬膜外瘢痕中TGF-β1和TGF-β2基因表达的影响 |
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投稿时间：2006-10-12
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| 作者 | Author | 单位 | Address | E-Mail |
| 周辉 |
ZHOU Hui |
杭州市中医院骨伤科,浙江杭州310007 |
Department of Orthopaedics and Traumatology,the Hospital of TCM of Hangzhou,Hangzhou 310007,Zhejiang,China |
hotstone615@126.com |
| 范希玲 |
FAN Xi-ling |
浙江大学附属第一医院整形美容科 |
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| 夏志敏 |
XIA Zhi-min |
杭州市中医院骨伤科,浙江杭州310007 |
Department of Orthopaedics and Traumatology,the Hospital of TCM of Hangzhou,Hangzhou 310007,Zhejiang,China |
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| 郑悦亮 |
ZHENG Yue-liang |
浙江省人民医院急诊科 |
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| 韩勇 |
HAN Yong |
杭州市中医院骨伤科,浙江杭州310007 |
Department of Orthopaedics and Traumatology,the Hospital of TCM of Hangzhou,Hangzhou 310007,Zhejiang,China |
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| 张政宏 |
ZHANG Zheng-hong |
杭州市中医院骨伤科,浙江杭州310007 |
Department of Orthopaedics and Traumatology,the Hospital of TCM of Hangzhou,Hangzhou 310007,Zhejiang,China |
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| 期刊信息：《中国骨伤》2007年，第20卷，第10期，第674-678页 |
| DOI：doi:10.3969/j.issn.1003-0034.yyyy.nn.zzz |
| 基金项目:杭州市医药卫生科技项目资助课题(编号:2003A024) |
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中文摘要:
目的:研究中药活血止痛汤对硬膜外瘢痕增生、粘连的预防作用,探讨其与硬膜外瘢痕形成过程中转化生长因子-β1(transforming growth factor,TGF-β1)和TGF-β2表达的关系,阐明其作用机制。
方法:雄性SD大鼠90只随机分为空白对照组(A组)、透明质酸钠组(B组)、活血止痛汤组(C组),每组30只。手术切除L4,5腰椎椎板,造成0.25cm×1cm硬脊膜裸露区,B组于硬膜囊外均匀地涂上一层透明质酸钠(约0.3ml),其余2组以等量生量盐水处理。术后第1天开始,C组予活血止痛汤2.5ml.kg-1.d-1灌胃;A、B组予以等量生理盐水,连用14d。术后第2、4、8周末处死动物,逆转录酶链反应(reverse transcription polymerase chain reaction,RT-PCR)测定其硬膜外瘢痕组织中TGF-β1和TGF-β2的表达水平,按Rydell法评定粘连程度,透射电镜观察其超微结构。
结果:第2、4、8周时,B、C组TGF-β1和TGF-β2的表达水平均明显低于A组(P<0.05),尤其在第8周时,C组TGF-β1和TGF-β2的表达水平与B组比较亦有统计学意义(P<0.05)。大体观察发现B、C组粘连程度明显减轻(P<0.05)。各时相B、C组瘢痕中成纤维细胞以及细胞内的细胞器的数量均减少,胶原纤维的合成受到抑制。
结论:活血止痛汤能通过下调TGF-β1和TGF-β2的表达,减少成纤维细胞增殖和胶原等细胞外基质(extracellularmatrix,ECM)的沉积,达到预防硬膜外瘢痕增生、粘连的效果。 |
| 【关键词】瘢痕组织 转化生长因子-β 中草药 大鼠 |
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| Effec of Huoxue Zhitong recipe（活血止痛汤）on the expression of TGF-β1 and TGF-β2 of the epidural scar tissue |
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ABSTRACT
Objective:To study the effect of Huoxue Zhitong recipe on the prevention of the epidural scar tissue adhesion and to explore the relationship with the expressions of transforming growth factor-β1(TGF-β1) and TGF-β2.
Methods:Ninety male SD rats were randomly divided into three groups,the control group A(rats=30),sodium hyaluronate group B(rats=30),Huoxue Zhitong recipe group C(rats=30).Laminectomy were performed at L4,5to expose a 0.25 cm×1 cm area of spinal dura,which were covered by 0.3 ml sodium hyaluronate in group B,or covered by same amount of saline in group A and C.At first day after operation,Rats were fed with Huoxue Zhitong recipe(2.5 ml·kg-1·d-1)by gavag in group C,and with same amount of saline in group A and B(continuous application for 14 d).The animals were sacrificed at the 2nd,4th and 8th weeks after operation.The specimens were prepared for determination of the expression of TGF-β1 and TGF-β2 at scar tissue and the degree of scar adhesion according to Rydell method.The ultrastructure of scar tissue was absened with transmission electron microscope(TEM).
Results:At the 2nd,4th,8th weeks after operation,the expression of TGF-β1 and TGF-β2 of group B and C were lower than that of group A(P<0.05).At the 8th week,the expression of the two cytokines in group C was significant decreased than that of group B(P<0.05).The duramater adhesion in group B and group C was less than that of group A(P<0.05).In group B and C,the amount of fibroblasts and intracellular organelle decreased,synthesis of collagen fiber was inhibited in different periods(P<0.05).
Conclusion:Huoxue Zhitong recipe can decrease the expression of TGF-β1 and TGF-β2and reduce proliferation of fibroblast and sedimentation of extracellular matrix,soit can prevent hyperplasia and adhesion for the scar tissue. |
| KEY WORDS Scar tissue Transforming growth factor-β Drugs,Chinese herbal Rats |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 周辉,范希玲,夏志敏,郑悦亮,韩勇,张政宏.活血止痛汤对硬膜外瘢痕中TGF-β1和TGF-β2基因表达的影响[J].中国骨伤,2007,20(10):674~678 |
| 英文格式: | ZHOU Hui,FAN Xi-ling,XIA Zhi-min,ZHENG Yue-liang,HAN Yong,ZHANG Zheng-hong.Effec of Huoxue Zhitong recipe（活血止痛汤）on the expression of TGF-β1 and TGF-β2 of the epidural scar tissue[J].zhongguo gu shang / China J Orthop Trauma ,2007,20(10):674~678 |
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