| 脉冲电磁场对去势大鼠骨髓源细胞体外培养破骨细胞变化的影响 |
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投稿时间：2009-04-16
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| 作者 | Author | 单位 | Address | E-Mail |
| 白孟海 |
BAI Meng-hai |
兰州军区兰州总医院骨科研究所,甘肃 兰州 730050 |
Institute of Orthopaedics,Lanzhou General Hospital of Lanzhou Military Area,Lanzhou 730050,Gansu,China |
Zhao6703@yahoo.com.cn |
| 葛宝丰 |
GE Bao-feng |
兰州军区兰州总医院骨科研究所,甘肃 兰州 730050 |
Institute of Orthopaedics,Lanzhou General Hospital of Lanzhou Military Area,Lanzhou 730050,Gansu,China |
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| 韦哲 |
WEI Zhe |
兰州军区兰州总医院骨科研究所,甘肃 兰州 730050 |
Institute of Orthopaedics,Lanzhou General Hospital of Lanzhou Military Area,Lanzhou 730050,Gansu,China |
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| 白洁 |
BAI Jie |
兰州军区兰州总医院安宁分院 |
Institute of Orthopaedics,Lanzhou General Hospital of Lanzhou Military Area,Lanzhou 730050,Gansu,China |
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| 程自峰 |
CHENG Zi-feng |
兰州军区兰州总医院骨科研究所,甘肃 兰州 730050 |
Institute of Orthopaedics,Lanzhou General Hospital of Lanzhou Military Area,Lanzhou 730050,Gansu,China |
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| 期刊信息：《中国骨伤》2009年，第22卷，第10期，第727-729页 |
| DOI：doi:10.3969/j.issn.1003-0034.yyyy.nn.zzz |
| 基金项目: |
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中文摘要:
目的：观察脉冲电磁场对去势大鼠骨髓源性破骨细胞样细胞(OLC)形成变化及凋亡的影响。
方法：30只健康3月龄雌性Wistar大鼠，随机分为A组(双侧卵巢切除+磁场治疗组、18只)、B组(双侧卵巢切除组、6只)和C组(伪去势组、6只).A组又随机均分3组：A1、A2、A3组，电磁场频率分别为1.5、2、75 Hz.术后单纯喂养3个月后对各组大鼠骨髓细胞进行培养，培养2 d后，A1、A2、A3组置脉冲电磁场治疗，1次/d,每次30 min,共治疗2周。之后各组培养细胞作瑞氏-吉姆萨、抗酒石酸酸性磷酸酶(TRAP)和Hoechst 33258染色，观察破骨细胞变化并计数。
结果：大鼠骨髓体外培养细胞TRAP染色结果显示，C组的破骨细胞形成数量最少，其次为A2、A3、A1、B组。B组的破骨细胞形成数量与C组和A2组比较显着增多(P<0.01),B组的破骨细胞形成数量与A1、A3组比较明显增多(P<0.05).Hoechst 33258染色结果显示，C组破骨样细胞凋亡出现数量较多，C、A2组凋亡数明显多于B组(P<0.05).
结论：脉冲电磁场能使大鼠骨髓体外培养破骨细胞形成数量减少、凋亡数增加，频率为2 Hz的效果最佳，表明脉冲电磁场可能将是骨质疏松症治疗的新方法。 |
| 【关键词】卵巢切除 大鼠 脉冲电磁场 破骨细胞 细胞凋亡 |
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| Effect of pulsed electromagnetic field on the changes of osteoclasts in ovariectomized rats bone marrow culture in vitro |
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ABSTRACT
Objective: To investigate the effect of pulsed electromagnetic fields(PEMs) on inducing osteoclastic like cell (OLC) formation changes and apoptosis in ovariectomized(OVX) rats bone marrow culture in vitro.
Methods: Thirty healthy three-month-old female Wistar rats were either sham-operated(Sham) or ovariectomized(OVX)and randomly divided into three groups:group A (OVX+PEMs,18 rats),group B (OVX,6 rats) and group C (Sham,6 rats);group A was again randomly divided into three groups:A1、A2、A3. The frequencies adopted were 1.5,2,75 Hz and 30 minutes for once a day. All rats were fed with normal diet for 3 months,then the bone marrow of all rats were cultured,2 days later,group A cells(including group A1,A2,A3) were collected and exposed to different frequencies PEMs for 2 weeks(30 min/day). In order to observe the changes of osteoclasts and count their numbers,cells were taken for Wright Giemsa staining,tartrate-resistance acid phosphatase(TRAP) staining and Hoechst 33258 staining.
Results: TRAP staining results indicated the number of OLC in group C was the least,then was group A2,A3,A1,B. The number of OLC in group B was remarkly increased(P<0.01;vs group C,A2). The number of OLC in group B was significantly increased(P<0.05;vs group A1,A3). Hoechst 33258 staining results indicated the number of apoptosis of OLC in group C was more than other groups,which of group C,A2 was significantly increased(P<0.05;vs group B).
Conclusion: PEMs had decreased the formation of OLC and increased the number of apoptosis of OLC in ovariectomized(OVX) rats bone marrow culture in vitro,the effects of 2 Hz was the best. PEMs would be a new way of osteoporosis therapy. |
| KEY WORDS Ovariectomy Rats Pulsed electromagnetic fields Osteoclasts Apoptosis |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 白孟海,葛宝丰,韦哲,白洁,程自峰.脉冲电磁场对去势大鼠骨髓源细胞体外培养破骨细胞变化的影响[J].中国骨伤,2009,22(10):727~729 |
| 英文格式: | BAI Meng-hai,GE Bao-feng,WEI Zhe,BAI Jie,CHENG Zi-feng.Effect of pulsed electromagnetic field on the changes of osteoclasts in ovariectomized rats bone marrow culture in vitro[J].zhongguo gu shang / China J Orthop Trauma ,2009,22(10):727~729 |
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