| 针刺对膝骨关节炎大鼠软骨白细胞介素-1β及肿瘤坏死因子-α表达的影响 |
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投稿时间：2011-05-08
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| 作者 | Author | 单位 | Address | E-Mail |
| 王道海 |
WANG Dao-hai |
中国医学科学院北京协和医学院北京协和医院，北京 100730 |
Peking Union Medical College Hospital, Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing 100730, China |
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| 包飞 |
BAO Fei |
中国医学科学院北京协和医学院北京协和医院，北京 100730 |
Peking Union Medical College Hospital, Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing 100730, China |
zengfei68@tom.com |
| 吴志宏 |
WU Zhi-hong |
中国医学科学院北京协和医学院北京协和医院，北京 100730 |
Peking Union Medical College Hospital, Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing 100730, China |
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| 孙华 |
SUN Hua |
中国医学科学院北京协和医学院北京协和医院，北京 100730 |
Peking Union Medical College Hospital, Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing 100730, China |
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| 张云祥 |
ZHANG Yun-xiang |
中国医学科学院北京协和医学院北京协和医院，北京 100730 |
Peking Union Medical College Hospital, Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing 100730, China |
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| 期刊信息：《中国骨伤》2011年，第24卷，第9期，第775-778页 |
| DOI：10.3969/j.issn.1003-0034.2011.09.019 |
| 基金项目:国家中医药管理局中医药科学技术研究专项课题(编号：06-07LP01) |
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中文摘要:
目的：探讨针刺对骨关节炎(OA)软骨中炎性细胞因子的影响作用。
方法：选用SD雌性大鼠40只，采用随机数字表，随机分为正常组、模型组、针刺组和对照组，各10只。采用单侧后肢跟腱切除法建立OA动物模型，切除左后肢跟腱，分别采用电针和扶他林乳剂对针刺组和对照组非手术侧(右后肢)进行治疗。采用免疫组化技术，观察各组关节软骨中白细胞介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)表达的特点，并比较各组间的差异。
结果：模型组IL-1β和TNF-α的表达都较正常组上调(P<0.01);模型组、针刺组和对照组3组比较，IL-1β和TNF-α的表达差异有统计学意义，针刺组和对照组的表达均较模型组下调(P<0.01),两者在针刺组与对照组的表达差异无统计学意义(P>0.05).
结论：针刺能下调OA软骨细胞IL-1β、TNF-α的表达，与扶他林乳剂作用相当，说明针刺对OA软骨具有一定的保护作用。 |
| 【关键词】骨关节炎 针刺 白细胞介素1β 肿瘤坏死因子α |
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| Influence of acupuncture on IL-1β and TNF-α expression in the cartilage of rats with knee osteoarthritis |
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ABSTRACT
Objective: To explore the influence of acupuncture treatment on proinflammatory cytokines of osteoarthritic(OA) cartilage.
Methods: Forty female SD rats were randomly and averagely divided into four groups:the normal,model,acupuncture and control group. The method of heel tendon resection for unilateral hind limb was used to establish OA animal models-that was to cut off the rat's heel tendon of left hind limb. The acupuncture group and control group were respectively treated by electro-acupuncture and Diclofenac on right hind limb of each animal for 2 weeks. Immunohistochemical staining was used to observe the expression characteristic of IL-1β and TNF-α in cartilage of each group,and the differences were compared among the groups.
Results: Both IL-1β and TNF-α expression in model group were significantly up-regulated than those in the normal group(P<0.01). Their expression in model,acupuncture and control group were significantly different(P<0.01). IL-1β and TNF-α expression were significantly down-regulated in acupuncture and control group than that in model group(P<0.01),while the difference between the two former groups was not significant(P>0.05).
Conclusion: Acupuncture can down-regulate the expression of IL-1β and TNF-α in osteoarthritic chondrocytes,and the regulating effect is the same as Diclofenac. This shows that acupuncture has a certain protective effect on osteoarthritic cartilage. |
| KEY WORDS Osteoarthritis Acupuncture Interleukin-1 beta Tumor necrosis factor-alpha |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 王道海,包飞,吴志宏,孙华,张云祥.针刺对膝骨关节炎大鼠软骨白细胞介素-1β及肿瘤坏死因子-α表达的影响[J].中国骨伤,2011,24(9):775~778 |
| 英文格式: | WANG Dao-hai,BAO Fei,WU Zhi-hong,SUN Hua,ZHANG Yun-xiang.Influence of acupuncture on IL-1β and TNF-α expression in the cartilage of rats with knee osteoarthritis[J].zhongguo gu shang / China J Orthop Trauma ,2011,24(9):775~778 |
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