| 健骨方对兔激素性股骨头坏死股骨头局部过氧化物酶体增殖物激活受体的影响 |
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投稿时间：2011-11-02
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| 期刊信息：《中国骨伤》2012年，第25卷，第5期，第407-410页 |
| DOI：10.3969/j.issn.1003-0034.2012.05.014 |
| 基金项目: |
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中文摘要:
目的：健骨方是临床上治疗激素性股骨头坏死的经验方,具有补肾活血、健脾化痰作用,主要由何首乌、泽泻、山楂、骨碎补、枸杞子、黄芪、地黄、丹参、当归、党参、女贞子、甘草等药物组成,本研究旨在探讨其对激素性股骨头坏死兔股骨头局部PPARγ影响。
方法：以18只健康SPF级新西兰家兔为研究对象,随机分成空白对照组、模型组、健骨方组。健骨方组予灌胃健骨方混悬液,每公斤体重10 ml,含生药0.719 g/ml;其余灌胃生理盐水,每公斤体重10 ml.3周后肌注甲泼尼龙珀酸钠,40 mg/kg,继续常规饲养3周后测定股骨头局部糖皮质激素水平、PPARγ以及造模前后血浆糖皮质激素水平。
结果：造模前血浆糖皮质激素水平组间差异无统计学意义(P=0.301).3周后,与空白对照组比较,模型组血浆糖皮质激素水平升高(P=0.001);与模型组比较,健骨方组血浆糖皮质激素水平降低(P=0.001).股骨头局部糖皮质激素含量,与空白对照组比较,模型组明显升高(P=0.001);与模型组比较,健骨方组降低(P=0.001).股骨头局部PPARγ含量,与空白对照组比较,模型组升高(P=0.018);与模型组比较,健骨方组降低(P=0.033).
结论：健骨方可能通过降低PPARγ含量抑制股骨头局部成脂分化,达到防治激素性股骨头坏死的作用。 |
| 【关键词】股骨头坏死 补肾 活血 过氧化物酶体增殖物激活受体 |
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| Influence of Jiangu decoction(健骨方) on peroxisome proliferator-activated receptor(PPAR)in the femoral head of rabbits with steroid-induced femoral head necrosis |
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ABSTRACT
Objective:The Jiangu decoction(健骨方) is used in the treatment of steroid-induced femoral head necrosis in clinical experiences,which has functions of tonifying kidney and activating blood,and invigorating spleen to remove phlegm. The decoction is mainly composed of Radix Polygoni Multiflori,Rhizoma alismatis Rhizoma Drynariae,haw,medlar,Radix Astragali,radix rehmanniae,angelica,Radix Codonopsis,radix salviae miltiorrhizae,Fructus Ligustri Lucidi,licorice,pharmaceutical composition. This study was designed to investigate the influence of Jiangu decoction(健骨方) on peroxisome proliferator-activated receptor γ(PPARγ)in the femoral head of rabbits with steroid-induced femoral head necrosis.
Methods:Eighteen adult SPF healthy New Zealand rabbits were divided into 3 groups:control group,model group,Jiangu decoction(健骨方) group. The rabbits of Jiangu decoction(健骨方) group orally received Jiangu decoction(健骨方) suspension with a dose of 10 ml/kg each day and the drug content was 0.719 g/ml. The rabbits in control and model groups were given saline with a dose of 10 ml/kg. The methylprednisolone sodium succinate was injected intramuscularly into left leg with a dose of 40 mg/kg. Then the rabbits were fed continuously for 3 weeks. The glucocorticoid levels,PPARγ and plasma glucocorticoid levels in the femoral head were measured before and after modeling.
Results:Before model established,the plasma glucocorticoid levels had no significant difference among three groups(P=0.301). At 3 weeks after model established,the plasma glucocorticoid level of rabbits in model group increased compared to the control group(P=0.001);and the plasma glucocorticoid level of rabbits in Jiangu decoction(健骨方)group decreased compared with model group(P=0.001). The glucocorticoid level in the local femoral head of rabbits in model group increased compared to the control group(P=0.001);and the glucocorticoid level in the local femoral head of rabbits in Jiangu decoction(健骨方) group decreased compared with model group(P=0.001). The PPARγ level in the local femoral head of rabbits in model group increased compared to the control group(P=0.018);and the PPARγ level in the local femoral head of rabbits in Jiangu decoction(健骨方) group decreased compared with model group(P=0.033).
Conclusion:The Jiangu decoction(健骨方) is effective to inhibit the femoral head adipogenic differentiation by decrease the PPAR content,so as to prevent and treat steroid-induced femoral head necrosis. |
| KEY WORDS Femur head necrosis Reinforcing kidney Activating blood Peroxisome proliferator-activated receptors |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 刘文刚,何伟,许学猛,吴淮,魏凌峰,赵传喜.健骨方对兔激素性股骨头坏死股骨头局部过氧化物酶体增殖物激活受体的影响[J].中国骨伤,2012,25(5):407~410 |
| 英文格式: | LIU Wen-gang,HE Wei,XU Xue-meng,WU Huai,WEI Ling-feng,ZHAO Chuan-xi.Influence of Jiangu decoction(健骨方) on peroxisome proliferator-activated receptor(PPAR)in the femoral head of rabbits with steroid-induced femoral head necrosis[J].zhongguo gu shang / China J Orthop Trauma ,2012,25(5):407~410 |
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