| 鹿茸多肽对实验性膝骨性关节炎软骨细胞凋亡及相关细胞因子的影响 |
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投稿时间：2011-08-20
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| 作者 | Author | 单位 | Address | E-Mail |
| 修忠标 |
XIU Zhong-biao |
福建中医药大学附属人民医院骨伤科,福建 福州 350004 |
Department of Orthopaedics,People's Hospital Affiliated to Fujian University of Traditional Chinese Medicine,Fuzhou 350004,Fujian,China |
xzbdoctor@sina.com |
| 孙磊 |
SUN Lei |
福建中医药大学附属人民医院骨伤科,福建 福州 350004 |
Department of Orthopaedics,People's Hospital Affiliated to Fujian University of Traditional Chinese Medicine,Fuzhou 350004,Fujian,China |
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| 期刊信息：《中国骨伤》2012年，第25卷，第5期，第418-423页 |
| DOI：10.3969/j.issn.1003-0034.2012.05.017 |
| 基金项目:福建省自然科学基金计划高校专项项目(No.C0740003) |
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中文摘要:
目的：探讨鹿茸多肽对实验性膝骨性关节炎软骨细胞凋亡及相关细胞因子的影响。
方法：6月龄新西兰大白兔64只,随机分为正常组(8只)和模型组(56只),模型组采用Hulth法造成兔膝骨性关节炎模型。造模成功后再将模型组随机分为鹿茸多肽组(24只)和对照组(24只),鹿茸多肽组予鹿茸多肽稀释液0.5 ml关节腔注射每2日1次,对照组给予0.5 ml生理盐水关节腔注射每2日1次。分别于干预后第7、15、30天分批取材,光镜观察各组关节软骨形态学变化,透射电镜观察软骨细胞凋亡的结构变化;TUNEL法检测软骨细胞凋亡,计算软骨细胞凋亡指数;酶联免疫吸附法检测关节液中白细胞介素-1β和肿瘤坏死因子-α的水平。
结果：随着时间的延长,鹿茸多肽组和对照组关节软骨退变进行性加重,软骨细胞凋亡明显增多。干预后第7、15、30天,鹿茸多肽组软骨细胞凋亡指数分别为(20.30±1.23)、(28.60±2.37)、(37.10±1.82),对照组软骨细胞凋亡指数分别为(31.50±2.44)、(34.40±1.77)、(42.30±2.33),差异有统计学意义(P<0.05);相同时间段内,鹿茸多肽组软骨细胞凋亡指数低于对照组。干预后第7、15、30天,鹿茸多肽组关节液中白细胞介素-1β水平分别为(15.81±1.26)、(12.59±1.42)、(9.57±0.92) μg/L,肿瘤坏死因子-α水平分别为(48.47±2.64)、(43.46±1.33)、(40.96±1.05) μg/L,差异有统计学意义(P<0.05).对照组关节液中白细胞介素-1β水平分别为(18.92±1.83)、(20.25±2.76)、(22.13±2.24) μg/L;肿瘤坏死因子-α水平分别为(57.92±2.12)、(60.25±1.48)、(63.35±2.15) μg/L.相同时间段内,鹿茸多肽组白细胞介素-1β和肿瘤坏死因子-α水平低于对照组,差异有统计学意义(P<0.05).
结论：鹿茸多肽可抑制膝骨性关节炎过程中软骨细胞凋亡,降低关节液中白细胞介素-1β和肿瘤坏死因子-α的水平,一定程度上延缓关节软骨的退变。 |
| 【关键词】骨关节炎,膝 鹿茸多肽 细胞凋亡 白细胞介素-1 肿瘤坏死因子-α |
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| Effects of Pilose antler polypeptide on apoptosis of chondrocyte and related cytokines in experimental knee osteoarthritis |
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ABSTRACT
Objective:To explore the effects of Pilose antler polypeptide on apoptosis of chondrocyte and related cytokines in experimental knee osteoarthritis.
Methods:Totally 64 New Zealand White rabbits of 6 months old were randomly divided into 2 groups:normal group(n=8)and model group (n=56). Model group was surgically induced into knee osteoarthritis model by method of Hulth. After successful modeling,the rabbits of model group were further divided into 2 groups:Pilose antler polypeptide-treatment group(n=24) and control group(n=24). Pilose antler polypeptide-treatment group received 0.5 ml intra-articular injection of Pilose antler polypeptide dilution liquid once per 2 days for 30 days while control group received 0.5 ml intra-articular injection of physiological saline. On days 7,15 and 30 after intervention,articular cartilage samples and synovial fluid were collected respectively. The morphological changes of articular cartilage under optical microscope and the structural change of chondrocyte were observed by transmission electron microscopy. The levels of interleukin-1β and tumor necrosis factor-α in synovial fluid was detected by Enzyme-linked Immunosorbent Assay.
Results:Along with the extending of time,articular cartilage degenerated gradually and chondrocytes apoptosis increased significantly. On days 7,15 and 30 after intervention,the chondrocyte apoptosis index of the Pilose antler polypeptide-treatment group were (20.30±1.23),(28.60±2.37),(37.10±1.82) and those of control group were(31.50±2.44),(34.40±1.77),(42.30±2.33). There were significant differences between them(P<0.05). At the same time,the chondrocyte apoptosis index of the Pilose antler polypeptide-treatment group were lower than those of control group,which had a statistical significance(P<0.05). On days 7,15 and 30 after intervention,the levels of interleukin-1β in synovia fluid of Pilose antler polypeptide-treatment group were(15.81±1.26),(12.59±1.42),(9.57±0.92) μg/L and the level of tumor necrosis factor-α were (48.47±2.64),(43.46±1.33),(40.96±1.05)μg/L,with statistical differences(P<0.05). The levels of interleukin-1β in synovia fluid of control group were(18.92±1.83),(20.25±2.76),(22.13±2.24)μg/L and the levels of tumor necrosis factor-α were (57.92±2.12),(60.25±1.48),(63.35±2.15) μg/L. At the same period,the levels of interleukin-1β and tumor necrosis factor-α were lower than those of the control group,which had a statistical significance(P<0.05).
Conclusion:Pilose antler polypeptide can inhibit chondrocytes apoptosis,decrease the levels of interleukin-1β and tumor necrosis factor-α and delay the degeneration of articular cartilage to some extent. |
| KEY WORDS Osteoarthritis,knee Pilose antler polypeptide Apoptosis Interleukin-1 Tumor necrosis factor-aplha |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 修忠标,孙磊.鹿茸多肽对实验性膝骨性关节炎软骨细胞凋亡及相关细胞因子的影响[J].中国骨伤,2012,25(5):418~423 |
| 英文格式: | XIU Zhong-biao,SUN Lei.Effects of Pilose antler polypeptide on apoptosis of chondrocyte and related cytokines in experimental knee osteoarthritis[J].zhongguo gu shang / China J Orthop Trauma ,2012,25(5):418~423 |
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