| 交锁髓内钉与钢板内同定微创治疗胫腓骨多段骨折的病例对照研究 |
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投稿时间：2014-05-01
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| 作者 | Author | 单位 | Address | E-Mail |
| 李念虎 |
LI Nian-hu |
山东中医药大学附属医院骨科, 山东 济南 250014 |
Department of Orthopaedics, Affiliated Hospital to Shandong University of TCM, Jinan 250014, Shandong, China |
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| 徐展望 |
XU Zhan-wang |
山东中医药大学附属医院骨科, 山东 济南 250014 |
Department of Orthopaedics, Affiliated Hospital to Shandong University of TCM, Jinan 250014, Shandong, China |
xzw6001@163.com |
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| 期刊信息：《中国骨伤》2015年，第28卷，第4期，第345-349页 |
| DOI：10.3969/j.issn.1003-0034.2015.04.012 |
| 基金项目:山东省自然科学基金项目(编号:Y2008C147) |
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中文摘要:
目的:采用小鼠气囊模型评价柚皮苷对聚甲基丙烯酸甲酯(Polymethylmethacrylate,PMMA)诱发的破骨细胞性骨溶解的作用。
方法:选取48只雌性8～10周龄Balb/c小鼠纳入研究,采用背部注入空气法在其中的32只小鼠中建立气囊模型,植入同种系小鼠颅骨(颅骨源自其余的16只小鼠).实验共分为4组(150 mg/kg柚皮苷治疗组、30 mg/kg柚皮苷治疗组、PBS空白对照组、DMSO载体对照组),每组8只动物。对两个柚皮苷治疗组和DMSO载体对照组的小鼠使用PMMA颗粒刺激,每组8只相应浓度进行处理,颗粒刺激第7天收集囊膜和囊内植入骨进行抗酒石酸酸性磷酸酶(Tartrate-resistant acid phosphatase,TRAP)染色、Ca2+释放以及改良Masson染色病理分析进行评价,观察柚皮苷的治疗作用。
结果:与DMSO载体组相比,柚皮苷治疗组降低了TRAP阳性细胞浸润的数量,差异有统计学意义(P<0.01);其中150 mg/kg浓度优于30 mg/kg浓度(8.90±1.75 vs 15.23±1.86).在气囊模型骨吸收冲洗液中,柚皮苷能降低钙的释放,特别是 150 mg/kg浓度组更为明显(P<0.05).改良的Masson染色显示柚皮苷可减少PMMA刺激所致的骨胶原的丢失,但150 mg/kg浓度组作用大于30 mg/kg浓度组。大体及病理切片显示两种浓度的柚皮苷显着降低PMMA刺激所致的炎性反应,表现为气囊厚度的减低和炎性细胞浸润数量的减少。
结论:柚皮苷抑制PMMA诱导的破骨细胞形成,有效缓解PMMA诱发的炎性反应以及随后发生的急性骨吸收。 |
| 【关键词】柚皮苷 破骨细胞形成 骨吸收 假体周围骨溶解 |
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| Naringin reduced polymethylmethacrylate-induced osteolysis in the mouse air sacs model |
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ABSTRACT
Objective:To evaluate the influence of naringin on PMMA-induced osteoclastic bone resorption using the mouse air sacs model.
Methods:Total 48 female Balb/c mices with the age of 8 to 10 weeks were chosen in the study. Air were injected into the back in 32 mices and formed the air sacs, 6 d later, the skulls (originated from other 16 mices) were implanted to the air sacs. Thirty-two animals were divided into naringin treatment group (with 2 concentrations of 150 mg/kg and 30 mg/kg), DMSO group and PBS blank group, 8 animals in each group. Polymethylmethacrylate (PMMA) particles were injected into the air sacs in naringin treatment groups and DMSO group so as to irritate inflammatory reaction. Naringin with 2 concentrations of 150 mg/kg and 30 mg/kg were dissolved in DMSO of 0.2 ml, and were injected into air sacs, respectively. In PBS black group, no stimulation with PMMA particles, only injected PBS, and in DMSO group, injected DMSO without naringin. Tartrate resistant acid phosphatase (TRAP), Ca2+ release, modified Masson stain and histological analysis were performed on the 7th day after stimulation.
Results:Compared with DMSO group, naringin treatment group's cellular infiltration decreased(P<0.01); concentration of 150 mg/kg was better than that of concentrations of 30 mg/kg (8.90±1.75 vs 15.23±1.86). Naringin can decrease calcium release in the lavage of the air sacs bone resorption model, especially obvious in naringin with concentration of 150 mg/kg. Naringin can ameliorate the inflammatory reaction and the subsequent bone resorption (including bone collagen loss, TRAP positive cells amount and so on) in air sacs with bone implant and PMMA particles. Naringin with concentration of 150 mg/kg appeared to be an optimal dosage to deliver the therapeutic effects.
Conclusion:Naringin inhibits PMMA-induced osteoclastogenesis and ameliorates the PMMA-associated inflammatory reaction and the subsequent bone resorption. |
| KEY WORDS Naringin Osteoclastogenesis Bone resorption Periprosthetic osteolysis |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 李念虎,徐展望.交锁髓内钉与钢板内同定微创治疗胫腓骨多段骨折的病例对照研究[J].中国骨伤,2015,28(4):345~349 |
| 英文格式: | LI Nian-hu,XU Zhan-wang.Naringin reduced polymethylmethacrylate-induced osteolysis in the mouse air sacs model[J].zhongguo gu shang / China J Orthop Trauma ,2015,28(4):345~349 |
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