| 急性脊髓损伤模型大鼠血清和脊髓的代谢组学研究 |
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投稿时间：2016-10-18
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| 作者 | Author | 单位 | Address | E-Mail |
| 胡华辉 |
HU Hua-hui |
浙江中医药大学, 浙江 杭州 310053 |
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| 黄小龙 |
HUANG Xiao-long |
浙江中医药大学, 浙江 杭州 310053 |
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| 全仁夫 |
QUAN Ren-fu |
杭州市萧山区中医院, 浙江 杭州 311201 |
Xiaoshan Hospital of Traditional Chinese Medicine, Hangzhou 311201, Zhejiang, China |
quanrenf@263.net |
| 杨宗保 |
YANG Zong-bao |
厦门大学中医系, 电子科学系, 福建省等离子体与磁共振研究重点实验室, 福建 厦门 361005 |
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| 许晶晶 |
XU Jing-jing |
厦门大学中医系, 电子科学系, 福建省等离子体与磁共振研究重点实验室, 福建 厦门 361005 |
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| 期刊信息：《中国骨伤》2017年，第30卷，第2期，第152-158页 |
| DOI：10.3969/j.issn.1003-0034.2017.02.012 |
| 基金项目:浙江省自然基金（编号:LY15H270003）；浙江省中医药科技计划项目（编号:2015ZZ017） |
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中文摘要:
目的：采用1H NMR核磁共振代谢组学的方法研究急性脊髓损伤模型大鼠的代谢组学特征及生物标志物，探讨核磁共振代谢组学应用于脊髓损伤研究的可行性。
方法：取8周龄清洁级雄性SD大鼠20只，体重（200±10）g，按照随机数字法分为假手术组和模型组，每组10只，模型组采用改良的Allens法制作急性脊髓不完全损伤模型，假手术组不损伤脊髓，术后第1、5、7天采用BBB运动功能评分法进行行为学观察，术后第7天收集脊髓组织作病理学观察，核磁共振代谢组学对两组大鼠血清和脊髓样本进行代谢组学分析。
结果：BBB评分显示假手术组术后后肢运动无明显改变，各时间点差异无统计学意义，模型组大鼠术后双下肢呈迟缓性瘫痪，BBB运动评分较低，各时间点差异存在统计学意义，两组运动功能评分在各时间点的差异均有统计学意义；病理切片显示假手术脊髓结构正常，神经分布均匀，模型组脊髓组织结构紊乱，神经元数目减少，存在炎性细胞浸润和空腔坏死组织。代谢组学分析表明，血清中极低密度脂蛋白（VLDL）、低密度脂蛋白（LDL）、谷氨酰胺（glutamine）、柠檬酸（citrate）、二甲基甘氨酸（DMG）等物质和脊髓中谷胱甘肽（glutathione）、3-羟基丁酸（3-OH-butyrate）、N-乙酰天冬氨酸（NAA）、磷酸胆碱（GPC）、谷氨酸（glutamate）、抗坏血酸（ascorbate）等物质浓度有明显变化（P<0.05）。
结论：通过对假手术组和模型组大鼠血清和脊髓样本进行代谢组学检测和分析得到了两组样本的差异性代谢物质，有助于解释急性脊髓损伤后血清和脊髓组织中的特异性小分子物质的变化规律，为后期针对性地研究这些代谢标记物在急性脊髓损伤中的作用提供研究基础。 |
| 【关键词】脊髓损伤 核磁共振 代谢组学 |
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| The metabolic profilings study of serum and spinal cord from acute spinal cord injury rats 1H NMR spectroscopy |
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ABSTRACT
Objective: To establish the rat model of acute spinal cord injury,followed by aprimary study on this model with 1H NMR based on metabonomics and to explore the metabonomics and biomarkers of spinal cord injury rat.
Methods: Twenty eight-week-old adult male SD rats of clean grade,with body weight of (200±10) g,were divided into sham operation group and model group in accordance with the law of random numbers,and every group had 10 rats. The rats of sham operation group were operated without damaging the spinal cord,and rats of model group were made an animal model of spinal cord incomplete injury according to the modified Allen's method. According to BBB score to observate the motor function of rats on the 1th,5th,and 7th days after surgery. Postoperative spinal cord tissue was collected in order to pathologic observation at the 7th day,and the metabolic profilings of serum and spinal cord from spinal cord injury rats were studied by 1H NMR spectroscopy.
Results: The hindlimb motion of rats did not obviously change in sham operation group,there was no significant difference at each time point;and rats of model group occurred flaccid paralysis of both lower extremities,there was a significant difference at each time; there was significant differences between two groups at each time. Pathological results showed the spinal cord structure was normal with uniform innervation in shame group,while in model group,the spinal cord structure was mussy,and the neurons were decreased,with inflammatory cells and necrotic tissue. Analysis of metabonomics showed that concentration of very low density fat protein (VLDL),low density fat protein (LDL),glutamine,citric acid,dimethylglycine (DMG) in the serum and glutathione,3-OH-butyrate,N-Acetyl-L-aspartic acid (NAA),glycerophosphocholine (GPC),glutamic acid,and ascorbate in spinal cord had significant changes(P<0.05).
Conclusion: There are significant differences in metabolic profile from serum and spinal cord sample between model group and sham operation group,it conduces to explain the changes of small molecular substances in serum and spinal cord tissue after spinal cord injury,this provides the research basis for targeted research on the role of metabolic markers in patients with acute spinal cord injury. |
| KEY WORDS Spinal cord injury Nuclear magnetic resonance Metabonomics |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 胡华辉,黄小龙,全仁夫,杨宗保,许晶晶.急性脊髓损伤模型大鼠血清和脊髓的代谢组学研究[J].中国骨伤,2017,30(2):152~158 |
| 英文格式: | HU Hua-hui,HUANG Xiao-long,QUAN Ren-fu,YANG Zong-bao,XU Jing-jing.The metabolic profilings study of serum and spinal cord from acute spinal cord injury rats 1H NMR spectroscopy[J].zhongguo gu shang / China J Orthop Trauma ,2017,30(2):152~158 |
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