50 Hz 1.8 mT正弦交变电磁场对生长期大鼠骨密度的影响研究
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作者Author单位AddressE-Mail
高玉海 GAO Yu-hai 兰州军区总医院全军创伤骨科研究所, 甘肃 兰州 730050 Institute of Orthopaedics, Lanzhou General Hospital of Lanzhou Military Area Command of Chinese PLA, Lanzhou 730050, Gansu, China  
周延峰 ZHOU Yan-feng 兰州军区总医院全军创伤骨科研究所, 甘肃 兰州 730050 Institute of Orthopaedics, Lanzhou General Hospital of Lanzhou Military Area Command of Chinese PLA, Lanzhou 730050, Gansu, China  
李少锋 LI Shao-feng 兰州军区总医院全军创伤骨科研究所, 甘肃 兰州 730050 Institute of Orthopaedics, Lanzhou General Hospital of Lanzhou Military Area Command of Chinese PLA, Lanzhou 730050, Gansu, China  
李文苑 LI Wen-yuan 兰州军区总医院全军创伤骨科研究所, 甘肃 兰州 730050 Institute of Orthopaedics, Lanzhou General Hospital of Lanzhou Military Area Command of Chinese PLA, Lanzhou 730050, Gansu, China  
葸慧荣 XI Hui-rong 兰州军区总医院全军创伤骨科研究所, 甘肃 兰州 730050 Institute of Orthopaedics, Lanzhou General Hospital of Lanzhou Military Area Command of Chinese PLA, Lanzhou 730050, Gansu, China  
杨芳芳 YANG Fang-fang 兰州军区总医院全军创伤骨科研究所, 甘肃 兰州 730050 Institute of Orthopaedics, Lanzhou General Hospital of Lanzhou Military Area Command of Chinese PLA, Lanzhou 730050, Gansu, China  
陈克明 CHEN Ke-ming 兰州军区总医院全军创伤骨科研究所, 甘肃 兰州 730050 Institute of Orthopaedics, Lanzhou General Hospital of Lanzhou Military Area Command of Chinese PLA, Lanzhou 730050, Gansu, China chkeming@lut.cn 
期刊信息:《中国骨伤》2017年,第30卷,第12期,第1113-1117页
DOI:10.3969/j.issn.1003-0034.2017.12.008
基金项目:国家自然科学基金(编号:81270963);国家国际科技合作专项项目(编号:2015DFR30940);甘肃省空间辐射生物学重点实验室开放基金(编号:2016GKL002)
中文摘要:

目的:探讨50 Hz 1.8 mT正弦交变电磁场(sinusoidal electromagnetic fields,SEMFs)对SD大鼠骨密度(bone mineral density,BMD)的影响。

方法:将30只1月龄体重为(110±10)g的SD大鼠随机分为正常对照组和电磁场组,各15只。分别给予对照组50 Hz 0 mT和电磁场组50 Hz 1.8 mT强度正弦交变电磁场干预,1.5 h/d,每周称取体重1次,每天监测进食量变化,6周后腹腔注射麻醉大鼠并用双能X骨密度检测全身骨密度,处死后检测股骨骨密度及椎体骨密度;ELISA法测定血清骨钙素(Osteocalcin,OC)和血清抗酒石酸性磷酸酶5b(tartrate-resistant acid phosphatase 5b,TRACP 5b);剥离肝、肾、子宫称重,计算器官指数并做HE切片做常规病理学检测分析。

结果:与对照组相比,电磁场组大鼠各周体重、每日进食量未见明显变化;2、4周后全身骨密度无明显变化,6周后全身骨密度及离体股骨和椎骨骨密度显著增加;血清OC表达量显著增加,血清TRACP 5b表达量显著降低;子宫、肝脏、脾脏HE染色未见病变,脏器指数无异常。

结论:50 Hz 1.8 mT正弦交变电磁场能通过提高骨形成降低骨吸收相关因子的表达,从而提高青年大鼠的峰值骨密度,为临床电磁场预防骨质疏松的研究奠定基础。
【关键词】电磁场  骨密度  大鼠
 
Effect of 50 Hz 1.8 mT sinusoidal electromagnetic fields on bone mineral density in growing rats
ABSTRACT  

Objective: To study effects of 50 Hz 1.8 mT sinusoidal electromagnetic fields (SEMFs) on bone mineral density (BMD) in SD rats.

Methods: Thirty SD rats weighted(110±10) and aged 1 month were randomly divided into control group and electromagnetic field group,15 in each group. Normal control group of 50 Hz 0 mT density and sinusoidal electromagnetic field group of 50 Hz 1.8 mT were performed respectively with 1.5 h/d and weighted weight once a week,and observed food-intake. Rats were anesthesia by intraperitoneal injection and dual energy X-ray absorptiometry were used to detect bone density of whole body,and detected bone density of femur and vertebral body. Osteocalcin and tartrate-resistant acid phosphatase 5b were detected by ELSA;weighted liver,kidney and uterus to calculate purtenance index,then detected pathologic results by HE.

Results: Compared with control group,there was no significant change in weight every week,food-intake every day; no obvious change of bone density of whole body at 2 and 4 weeks,however bone density of whole body,bone density of excised femur and vertebra were increased at 6 weeks. Expression of OC was increased,and TRACP 5b expression was decreased. No change of HE has been observed in liver,kidney and uterus and organic index.

Conclusion: 50 Hz 1.8 mT sinusoidal electromagnetic fields could improve bone formation to decrease relevant factors of bone absorbs,to improve peak bone density of young rats,in further provide a basis for clinical research electromagnetic fields preventing osteoporosis foundation.
KEY WORDS  Electromagnetic fields  Bone density  Rats
 
引用本文,请按以下格式著录参考文献:
中文格式:高玉海,周延峰,李少锋,李文苑,葸慧荣,杨芳芳,陈克明.50 Hz 1.8 mT正弦交变电磁场对生长期大鼠骨密度的影响研究[J].中国骨伤,2017,30(12):1113~1117
英文格式:GAO Yu-hai,ZHOU Yan-feng,LI Shao-feng,LI Wen-yuan,XI Hui-rong,YANG Fang-fang,CHEN Ke-ming.Effect of 50 Hz 1.8 mT sinusoidal electromagnetic fields on bone mineral density in growing rats[J].zhongguo gu shang / China J Orthop Trauma ,2017,30(12):1113~1117
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