| 温针对肌筋膜痛扳机点模型大鼠病理形态及致痛性炎性介质的影响 |
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投稿时间：2018-11-20
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| 作者 | Author | 单位 | Address | E-Mail |
| 温干军 |
WEN Gan-jun |
东莞市常平医院, 广东 东莞 523573 |
Changping Hospital of Dongguan City, Dongguan 523573, Guangdong, China |
413697236@qq.com |
| 刘红 |
LIU Hong |
东莞市常平医院, 广东 东莞 523573 |
Changping Hospital of Dongguan City, Dongguan 523573, Guangdong, China |
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| 陈坚 |
CHEN Jian |
东莞市常平医院, 广东 东莞 523573 |
Changping Hospital of Dongguan City, Dongguan 523573, Guangdong, China |
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| 张史飞 |
ZHANG Shi-fei |
东莞市常平医院, 广东 东莞 523573 |
Changping Hospital of Dongguan City, Dongguan 523573, Guangdong, China |
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| 李义凯 |
LI Yi-kai |
南方医科大学中医药学院, 广东 广州 510515 |
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| 周树根 |
ZHOU Shu-gen |
东莞市常平医院, 广东 东莞 523573 |
Changping Hospital of Dongguan City, Dongguan 523573, Guangdong, China |
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| 期刊信息：《中国骨伤》2019年，第32卷，第3期，第260-264页 |
| DOI：10.3969/j.issn.1003-0034.2019.03.013 |
| 基金项目:东莞市科技局一般项目（编号：201750715035083） |
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中文摘要:
目的：在建立扳机点大鼠模型的基础上用温针进行干预，通过显微病理技术及微透析技术，评价其对扳机点模型大鼠病理形态及致痛炎性介质的影响。
方法：将64只SD雄性大鼠随机分为A组（空白对照）、B组（模型对照）和C组（模型干预）；A、B每组又分3小组（A0，A1，A2和B0，B1，B2），C组分为2小组（C1，C2）。B、C两组建立MTrPs模型，造模成功后并分别给予C1组7 d和C2组15 d温针干预。分批处死大鼠，MTrPs局部取材，制片后进行苏木精-伊红染色，光镜下观察其病理变化，通过微透析技术对大鼠动物模型扳机点局部白细胞介素-1β和前列素E2进行检测。
结果：镜下可见，造模组的肌纤维排列紊乱、断裂、扭曲，局部肌纤维纤维化、挛缩增粗等；局部可见有巨噬细胞等炎症细胞侵入及挛缩结节部位出现大面积粘连，温针干预后局部肌纤维的病理状态明显改善，局部微小血管形成和成熟，局部肌纤维修复。在造模成功后，进行温针干预前B0组的白细胞介素-1β和前列素E2的量明显高于A0组（P<0.01），通过7 d的温针干预，C1和B1组白细胞介素-1β和前列素E2的量差异无统计学意义（P>0.05），C1、B1组明显高于A1组（P<0.01）；通过15 d温针干预，C2组的白细胞介素-1β和前列素E2的量低于B2组（P<0.05），但C2、B2组明显高于A2组（P<0.01），C2组的白细胞介素-1β及前列素E2的量低于C1组（P<0.05）。
结论：运动联合击打的造模方式通过病理组织学证实是有效的；温针能够改善大鼠肌筋膜痛扳机点局部肌纤维的病理状态及炎症状态，促进局部微小血管形成和成熟，有助于扳机点局部肌纤维修复。 |
| 【关键词】温针 肌筋膜痛 扳机点 病理形态 炎性介质 |
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| Effect of warm acupuncture on pathological morphology and pain-induced inflammatory mediators in rats with myofascial pain trigger |
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ABSTRACT
Objective:Based on the establishment of a rat model of trigger point,this study was to intervene with warm acupuncture,and to evaluate the effect on pathological morphology and pain-induced inflammation of the rat model by microscopic pathology and microdialysis.
MethodsSixty-four SD rats were randomly divided into group A (blank control),group B (model control) and group C (model and intervention control). Groups A and B were divided into 3 groups (A0,A1,A2 and B0,B1,B2),the group C was divided into 2 groups (C1 and C2). The MTrPs model was established in both groups B and C,warm acupuncture intervention were given to the C1 group for 7 days and the C2 group for 15 days. Rats were sacrificed in batches. MTrPs were locally sampled and stained with hematoxylin-eosin after the preparation. The pathological changes were observed under light microscopy. The iocal interleukin-1β and prostaglandin E2 were detected by microdialysis technique.
ResultsMicroscopically,the muscle fibers of the model were arranged disorderly,broken,twisted,local fibrosis,contracture thickening and so on;macrophage and other inflammatory cell invasion in local area and a large area of adhesion occurred on the contracture nodule,the pathological state of local muscle fibers was significantly improved after warm needle intervention,local microvascular formation and maturation,local muscle fiber repair. After successful modeling,the amount of interleukin-1β and prostaglandin E2 in group B0 was significantly higher than that in group A0 before warm needle intervention (P<0.01). After warming intervention for 7 days,there was no significant difference in the amount of interleukin-1β and prostaglandin E2 between group C1 and group B1 (P>0.05). Group C1 and B1 were significantly higher than group A1 (P<0.01);warm needle intervention for 15 days,the amount of interleukin-1β and prostaglandin E2 in group C2 were lower than those in group B2 (P<0.05),but those in group C2 and B2 were significantly higher than group A2 (P<0.01),and the amount of interleukin-1β and prostaglandin E2 in group C2 was lower than group C1 (P<0.05).
ConclusionThe modeling method of exercise combined hitting used in this study was proved to be effective by histopathology;warm acupuncture can improve the pathological and inflammatory state of local muscle fiber in myofascial pain trigger of rat,promote local microvascular formation and maturation,and help the trigger point local muscle fiber repair. |
| KEY WORDS Warm acupuncture Myofascial pain Trigger point Pathological morphology Inflammatory mediator |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 温干军,刘红,陈坚,张史飞,李义凯,周树根.温针对肌筋膜痛扳机点模型大鼠病理形态及致痛性炎性介质的影响[J].中国骨伤,2019,32(3):260~264 |
| 英文格式: | WEN Gan-jun,LIU Hong,CHEN Jian,ZHANG Shi-fei,LI Yi-kai,ZHOU Shu-gen.Effect of warm acupuncture on pathological morphology and pain-induced inflammatory mediators in rats with myofascial pain trigger[J].zhongguo gu shang / China J Orthop Trauma ,2019,32(3):260~264 |
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