| 铁蓄积骨质疏松模型凝血状态及微血栓和血管密度变化的研究 |
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投稿时间：2020-06-20
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| 作者 | Author | 单位 | Address | E-Mail |
| 杭海峰 |
HANG Hai-feng |
苏州大学附属第二医院, 江苏 苏州 215004
扬州市江都人民医院, 江苏 扬州 225200 |
Department of Orthopaedics, the Second Affiliated Hospital of Soochow University, Suzhou 215004, Jiangsu, China |
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| 徐又佳 |
XU You-jia |
苏州大学附属第二医院, 江苏 苏州 215004 |
Department of Orthopaedics, the Second Affiliated Hospital of Soochow University, Suzhou 215004, Jiangsu, China |
xuyoujia@suda.edu.cn |
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| 期刊信息：《中国骨伤》2020年，第33卷，第10期，第954-959页 |
| DOI：10.12200/j.issn.1003-0034.2020.10.013 |
| 基金项目:国家自然科学基金（编号：81874018）；苏州市民生科技项目（编号：SS201814）；江苏省临床医学科技专项（编号：BE2019661） |
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中文摘要:
目的：了解铁蓄积骨质疏松模型中凝血状态、微血栓、微血管床及骨密度的变化，探讨骨质疏松范畴铁蓄积对凝血功能、血管方面的影响。
方法：选取健康24只6月龄雄性SPF级SD大鼠，体重（250±20）g，随机分为对照组和铁蓄积组，每组12只。铁蓄积组用枸橼酸铁（ferric ammonium citrate，FAC）90 mg/kg腹腔注射干预，对照组采用等体积生理盐水腹腔注射，每周2次，干预9周。干预完成后，检测两组血清铁蛋白、凝血功能、微血栓、血管密度及股骨远端骨小梁三维形态重建和空间结构参数，并进行统计学分析。
结果：铁蓄积组血清铁蛋白（136.36±35.41）μg/L较对照组（68.44±16.86）μg/L显著升高，铁蓄积组骨密度（0.167±0.024）g/cm3较对照组（0.400±0.030）g/cm3显著下降；铁蓄积组的纤维蛋白原（2.03±0.13）g/L较对照组（1.78±0.46）g/L明显升高，D-二聚体含量（534.95±31.81）ng/ml较对照组（329.02±84.99）ng/ml明显增高（P<0.05），而凝血酶时间（39.64±2.18）s和凝血酶原时间（8.70±0.39）s较对照组（44.92±2.98）s、（9.44±0.49）s明显缩短（P<0.05）；墨汁染色后，铁蓄积组的微血管密度（17.46±2.07）%较对照组（23.81±2.98）%明显缩小（P<0.05）。HE和MSB染色均显示铁蓄积大鼠骨髓中存在微血栓，同时在心肌中也存在微血栓。
结论：在铁蓄积影响的骨质疏松模型中，铁蓄积对凝血功能有显著影响，血液相对呈高凝状态，骨血管床减少，骨髓中有微血栓存在，血液的高凝状态及微血栓的形成可能是铁蓄积骨质疏松症发生的重要影响因素。 |
| 【关键词】骨质疏松 铁 血栓 微循环 |
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| Study on changes of coagulation state,microthrombus and vascular density in the model of iron accumulation osteoporosis |
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ABSTRACT
Objective: To understand changes of coagulation state,microthrombus,microvascular bed and bone density in the osteoporosis model of iron accumulation,and explore the influence of iron accumulation in aspects of osteoporosis on coagulation function and blood vessels.
Methods: Tewnty-four male SPF SD rats aged 6 months were selected,which with the average body weight(250±20) g,which were divided into control group and iron accumulation group according to random number table,12 rats in each group. Iron accumulation group was intervened by intraperitoneal injection of ferric ammonium citrate 90 mg/kg,and control group was intraperitoneally injected with equal volume of normal saline,twice a week for 9 weeks. After intervention,serum ferritin,coagulation function,microthrombus,vascular density,and three-dimensional morphological reconstruction and spatial structure parameters of the distal femur trabeculae were measured and statistically analyzed.
Results: Serum ferritin of iron accumulation group (136.36±35.41) μg/L was higher than control group (68.44±16.86) μg/L(P<0.05). Bone mineral density (BMD) of iron accumulation group (0.167±0.024) g/cm3 was lower than control group(0.400±0.030)g/cm3. Fibrinogen of iron accumulation group(2.03±0.13) g/L was increased than that of control group(1.78±0.46) g/L,D-dimer contents of iron accumulation group(534.95±31.81) ng/ml was increased than that of control group(329.02±84.99) ng/ml,while thrombin time (39.64±2.18) s and prothrombin time(8.70±0.39) s of iron accumulation group were shorter than that of control group(44.92±2.98) s,(9.44±0.49) s(P<0.05). After ink staining,microvessel density in iron accumulation group(17.46±2.07)% was significantly reduced compared with that of control group(23.81±2.98)%(P<0.05). HE and MSB staining which showed microthrombus in bone marrow of iron accumulation rats,as well as microthrombus in myocardium.
Conclusion: In the osteoporosis model with the influence of iron accumulation,iron accumulation had a significant influence on the coagulation function,and the blood was relatively hypercoagulable. The bone vascular bed uas reduced,and there were microthrombus in the bone marrow. Hypercoagulable state of blood and formation of microthrombi may be important factors influencing the occurrence of iron accumulation osteoporosis. |
| KEY WORDS Osteoporosis Iron Thrombosis Microcirculation |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 杭海峰,徐又佳.铁蓄积骨质疏松模型凝血状态及微血栓和血管密度变化的研究[J].中国骨伤,2020,33(10):954~959 |
| 英文格式: | HANG Hai-feng,XU You-jia.Study on changes of coagulation state,microthrombus and vascular density in the model of iron accumulation osteoporosis[J].zhongguo gu shang / China J Orthop Trauma ,2020,33(10):954~959 |
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