| 骨肉瘤与尤文肉瘤关键基因CDC5L及其生物学功能的生物信息学分析 |
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投稿时间：2021-01-21
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| 作者 | Author | 单位 | Address | E-Mail |
| 冯娜 |
FENG Na |
吉林大学第一医院骨关节外科及运动医学护理平台, 吉林 长春 130021 |
Department of Sports Medicine, the First Hospital of Jilin University, Changchun 130021, Jilin, China |
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| 宋文霞 |
SONG Wen-xia |
吉林大学第一医院病理科, 吉林 长春 130021 |
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| 邵立平 |
SHAO Li-ping |
吉林大学第一医院骨关节外科及运动医学护理平台, 吉林 长春 130021 |
Department of Sports Medicine, the First Hospital of Jilin University, Changchun 130021, Jilin, China |
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| 高宇航 |
GAO Yu-hang |
吉林大学第一医院骨关节外科, 吉林 长春 130021 |
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| 赵承武 |
ZHAO Cheng-wu |
吉林大学第一医院运动医学科, 吉林 长春 130021 |
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chengwuzhao@jlu.edu.cn |
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| 期刊信息：《中国骨伤》2022年，第35卷，第3期，第276-280页 |
| DOI：10.12200/j.issn.1003-0034.2022.03.015 |
| 基金项目: |
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中文摘要:
目的: 利用生物信息学方法筛选骨肉瘤（osteosarcoma，OS）和尤文肉瘤（Ewing's sarcoma，EWS）关键基因及通路预测。
方法: 检索GEO数据库OS（GSE16088） 及EWS（GSE45544）数据集，利用GEO2R分析差异基因，韦恩图筛选共同参与两者发生的关键基因，对该基因进行GO、KEGG通路分析及蛋白质-蛋白质相互作用（protein-protein interaction，PPI）网络分析，并利用UCSC进行该基因的转录因子预测，GEPIA分析转录因子与目的基因之间相关性。
结果: 通过对OS及EWS数据集差异性及维恩图分析，结果显示细胞分裂周期5样蛋白（cell division cycke 5-like，CDC5L）基因为参与OS及EWS发病的关键基因。该基因主要参与有丝分裂过程，与RNA代谢、Pre-mRNA内含子的处理、mRNA及Pre-mRNA的剪接等信号通路。
结论: CDC5L作为关键基因，在 OS及EWS进展中发挥促癌基因的作用，可能是两种原发恶性肿瘤可靠的诊疗靶点。 |
| 【关键词】骨肉瘤 尤文肉瘤 生物信息学分析 CDC5L |
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| Bioinformatics-based identification of key genes CDC5L and related pathways in osteosarcoma and Ewing's sarcoma |
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ABSTRACT
Objective: Osteosarcoma(OS) and Ewing's sarcoma (EWS) are the two most common primary malignant bone tumors in children. The aim of the study was to identify key genes in OS and EWS and investigate their potential pathways.
Methods: Expression profiling (GSE16088 and GSE45544) were obtained from GEO DataSets. Differentially expressed genes were identified using GEO2R and key genes involved in the occurrence of both OS and EWS were selected using venn diagram. Gene ontology and pathway enrichment analyses were performed for the ensembl. Protein-protein interaction (PPI) networks were established by STRING. Further,UCSC was used to predict the transcription factors of the cell division cycke 5-like(CDC5L) gene,and GEPIA was used to analyze the correlation between the transcription factors and the CDC5L gene.
Results: The results showed that CDC5L gene was the key gene involved in the pathogenesis of OS and EWS. The gene is mainly involved in mitosis,and is related to RNA metabolism,processing of capped intron-containing pre-mRNA,mRNA and pre-mRNA splicing.
Conclusion: CDC5L,as a key gene,plays a role in development of OS and EWS,which may be reliable targets for diagnosis and treatment of these primary malignant tumors. |
| KEY WORDS Osteosarcoma Ewing's sarcoma Bioinformatics CDC5L |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 冯娜,宋文霞,邵立平,高宇航,赵承武.骨肉瘤与尤文肉瘤关键基因CDC5L及其生物学功能的生物信息学分析[J].中国骨伤,2022,35(3):276~280 |
| 英文格式: | FENG Na,SONG Wen-xia,SHAO Li-ping,GAO Yu-hang,ZHAO Cheng-wu.Bioinformatics-based identification of key genes CDC5L and related pathways in osteosarcoma and Ewing's sarcoma[J].zhongguo gu shang / China J Orthop Trauma ,2022,35(3):276~280 |
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